Information for Peptide: TIETSPSLSR from FANCI_HUMAN

1. Biological Peptide

1.1 Native Sequence

    -.TIETSPSLSR.A

1.2 Peptide Properties

Start	Stop	MW	m/z for 2+	m/z for 3+	pI
0	9	1089.57	545.79	364.2	5.66

1.3 Fragment Ion Table

AA	b Ion Series	b Ion Mass	y Ion Series	y Ion Mass
T	b		y
E	b		y
I	b		y
T	b		y
S	b		y
P	b		y
S	b		y
L	b		y
S	b		y
R	b		y

2. Synthetic Peptide

2.1 Synthetic (Internal Standard) Peptide Sequence

TEITSPSL(C6N1)SR (Modification: Stable Isotope on L8 (U-13C6, 15N) )

2.2 Peptide Properties

MW	m/z for 2+	m/z for 3+	pI
1096.58	549.3	366.54	5.66

2.3 Fragment Ion Table

AA	b Ion Series	b Ion Mass	y Ion Series	y Ion Mass
T	b		y
E	b		y
I	b		y
T	b		y
S	b		y
P	b		y
S	b		y
L	b		y
S	b		y
R	b		y

2.4 Peptide Synthesis Report

Click to view/download synthesis report for TIETSPSLSR

2.5 QqQ MS/MS

Transition Table:

Transition	m/z of Transition	Collision Energy
y5	566.32	22.0
y6	653.35	22.0
y7	754.4	22.0
y8	867.48	22.0

2.6 Calibration Curve of Standard

Description: A dilution series was done on the internal standards with concentrations ranging from 50 fmol to 0.1 fmol. Peptide sensitivity was reliable down to 0.1 fmol.

 

Experiment Description: Cells from 8226 line were lysed in RIPA buffer. The equivalent of 100,000 cells were loaded onto an SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/4 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer.

2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times

2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides