Information for Peptide: TELLPGDR from IBP7_HUMAN
1. Biological Peptide
1.1 Native Sequence
R.TELLPGDR.D
1.2 Peptide Properties
| Start | Stop | MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|---|---|
| 206 | 213 | 899.47 | 450.74 | 300.83 | 4.37 |
1.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| T | b | y | ||
| E | b | y | ||
| L | b | y | ||
| L | b | y | ||
| P | b | y | ||
| G | b | y | ||
| D | b | y | ||
| R | b | y |
2. Synthetic Peptide
2.1 Synthetic (Internal Standard) Peptide Sequence
TELLPGER (Modification: D2 switched to E )
2.2 Peptide Properties
| MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|
| 913.49 | 457.75 | 305.5 | 4.53 |
2.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| T | b | y | ||
| E | b | y | ||
| L | b | y | ||
| L | b | y | ||
| P | b | y | ||
| G | b | y | ||
| E | b | y | ||
| R | b | y |
2.4 Peptide Synthesis Report
Click to view/download synthesis report for TELLPGDR
2.5 QqQ MS/MS
Transition Table:
| Transition | m/z of Transition | Collision Energy |
|---|---|---|
| y4 | 458.2364 | 18.6 |
| y5 | 571.3204 | 18.6 |
| y6 | 684.4045 | 18.6 |
| y7 | 813.4471 | 18.6 |
2.6 Calibration Curve of Standard
Description: NONE
Experiment Description: Control urine was processed via ultrafiltration according to Moffitt Proteomics protocol and subjected to in-solution digestion with trypsin, following thermal denaturation, reduction, and alkylation.
2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times
2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides
