Information for Peptide: STETVDSGYR from AXN2_HUMAN
1. Biological Peptide
1.1 Native Sequence
R.STETVDSGYR.S
1.2 Peptide Properties
| Start | Stop | MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|---|---|
| 263 | 272 | 1113.49 | 557.75 | 372.17 | 4.37 |
1.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| S | b | y | ||
| T | b | y | ||
| E | b | y | ||
| T | b | y | ||
| V | b | y | ||
| D | b | y | ||
| S | b | y | ||
| G | b | y | ||
| Y | b | y | ||
| R | b | y |
2. Synthetic Peptide
2.1 Synthetic (Internal Standard) Peptide Sequence
STETVDSAYR (Modification: G8 to A )
2.2 Peptide Properties
| MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|
| 1127.51 | 564.76 | 376.84 | 4.37 |
2.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| S | b | y | ||
| T | b | y | ||
| E | b | y | ||
| T | b | y | ||
| V | b | y | ||
| D | b | y | ||
| S | b | y | ||
| A | b | y | ||
| Y | b | y | ||
| R | b | y |
2.4 Peptide Synthesis Report
Click to view/download synthesis report for STETVDSGYR
2.5 QqQ MS/MS
Transition Table:
| Transition | m/z of Transition | Collision Energy |
|---|---|---|
| y3 | 409.22 | 23.0 |
| y4 | 496.25 | 23.0 |
| y5 | 611.28 | 23.0 |
| y8 | 940.44 | 23.0 |
2.6 Calibration Curve of Standard
Description: Internal standard was diluted from 50 fmol to 500 amol injection concentrations and was found to be sensitive down to 0.1 fmol
Experiment Description: Cells from colon caner line HCT 116 were lysed in 8M urea/ 100mM ammonium bicarbonate buffer on ice. The equivalent of 100,000 cells were loaded onto a SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/4 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer. Internal standard has been synthesized and is awaiting analysis with sample.
2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times
2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides
