Information for Peptide: SPPPESVDTPTSTK from KI67_HUMAN
1. Biological Peptide
1.1 Native Sequence
K.SPPPESVDTPTSTK.Q
1.2 Peptide Properties
| Start | Stop | MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|---|---|
| 1131 | 1144 | 1441.69 | 721.85 | 481.57 | 4.37 |
1.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| S | b | y | ||
| P | b | y | ||
| P | b | y | ||
| P | b | y | ||
| E | b | y | ||
| S | b | y | ||
| V | b | y | ||
| D | b | y | ||
| T | b | y | ||
| P | b | y | ||
| T | b | y | ||
| S | b | y | ||
| T | b | y | ||
| K | b | y |
2. Synthetic Peptide
2.1 Synthetic (Internal Standard) Peptide Sequence
SPPPESVDTP(C5N1)TSTK (Modification: Stable Isotope on P10 (U-13C5, 15N) )
2.2 Peptide Properties
| MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|
| 1447.71 | 724.86 | 483.58 | 4.37 |
2.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| S | b | y | ||
| P | b | y | ||
| P | b | y | ||
| P | b | y | ||
| E | b | y | ||
| S | b | y | ||
| V | b | y | ||
| D | b | y | ||
| T | b | y | ||
| P | b | y | ||
| T | b | y | ||
| S | b | y | ||
| T | b | y | ||
| K | b | y |
2.4 Peptide Synthesis Report
Click to view/download synthesis report for SPPPESVDTPTSTK
2.5 QqQ MS/MS
Transition Table:
| Transition | m/z of Transition | Collision Energy |
|---|---|---|
| y4 | 436.24 | 28.0 |
| y5 | 539.29 | 28.0 |
| y6 | 640.34 | 28.0 |
| y7 | 755.37 | 28.0 |
| y8 | 854.44 | 28.0 |
| y10 | 1070.51 | 28.0 |
2.6 Calibration Curve of Standard
Description: NONE
Experiment Description: Cells from Breast cancer MCF7 cell line were lysed in 8M urea/ 100mM ammonium bicarbonate buffer on ice. The equivalent of 100,000 cells were loaded onto a SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/4 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer. Internal standard has been synthesized and is awaiting analysis with sample.
2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times
2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides
