Information for Peptide: SLEIEPR from SOS2_HUMAN

1. Biological Peptide

1.1 Native Sequence

    K.SLEIEPR.N

1.2 Peptide Properties

Start	Stop	MW	m/z for 2+	m/z for 3+	pI
1011	1017	842.45	422.23	281.82	4.53

1.3 Fragment Ion Table

AA	b Ion Series	b Ion Mass	y Ion Series	y Ion Mass
S	b		y
L	b		y
E	b		y
I	b		y
E	b		y
P	b		y
R	b		y

2. Synthetic Peptide

2.1 Synthetic (Internal Standard) Peptide Sequence

(NONE)

2.2 Peptide Properties

(NONE)

2.3 Fragment Ion Table

(NONE)

2.4 Peptide Synthesis Report

(NONE)

2.5 QqQ MS/MS

2.6 Calibration Curve of Standard

Description: NONE

 

Experiment Description: Cells from the Melanoma cancer cell line WM3670 were lysed in RIPA buffer. The equivalent of 200,000 cells were loaded onto an SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/6 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer.

2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times

2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides