Information for Peptide: SDALNSAIDK from CTNA1_HUMAN

1. Biological Peptide

1.1 Native Sequence

    R.SDALNSAIDK.M

1.2 Peptide Properties

Start	Stop	MW	m/z for 2+	m/z for 3+	pI
361	370	1032.51	517.26	345.18	4.21

1.3 Fragment Ion Table

AA	b Ion Series	b Ion Mass	y Ion Series	y Ion Mass
S	b		y
D	b		y
A	b		y
L	b		y
N	b		y
S	b		y
A	b		y
I	b		y
D	b		y
K	b		y

2. Synthetic Peptide

2.1 Synthetic (Internal Standard) Peptide Sequence

SDALNSGIDK (Modification: A7 to G )

2.2 Peptide Properties

MW	m/z for 2+	m/z for 3+	pI
1018.49	510.25	340.51	4.21

2.3 Fragment Ion Table

AA	b Ion Series	b Ion Mass	y Ion Series	y Ion Mass
S	b		y
D	b		y
A	b		y
L	b		y
N	b		y
S	b		y
G	b		y
I	b		y
D	b		y
K	b		y

2.4 Peptide Synthesis Report

Click to view/download synthesis report for SDALNSAIDK

2.5 QqQ MS/MS

Transition Table:

Transition	m/z of Transition	Collision Energy
y9	932.47	20.0
y8	817.44	19.0
y7	746.4	20.0
y6	633.32	18.0
y5	519.28	19.0
y4	432.25	26.0
y3	375.22	27.0

2.6 Calibration Curve of Standard

Description: Internal standard DAFFGNPR was diluted from 50 fmol to 500 amol injection concentrations and was found to be sensitive down to 0.1 fmol

 

Experiment Description: Cells from colon caner line HCT 116 were lysed in 8M urea/ 100mM ammonium bicarbonate buffer on ice. The equivalent of 100,000 cells were loaded onto a SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/4 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer. Sample was analyzed concurrently with internal standard

2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times

2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides