Information for Peptide: RPAGSVQNPVYHNQPLNPAPSR from EGFR_HUMAN
1. Biological Peptide
1.1 Native Sequence
K.RPAGSVQNPVYHNQPLNPAPSR.D
1.2 Peptide Properties
| Start | Stop | MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|---|---|
| 1100 | 1121 | 2398.23 | 1200.12 | 800.42 | 10.84 |
1.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| R | b | y | ||
| P | b | y | ||
| A | b | y | ||
| G | b | y | ||
| S | b | y | ||
| V | b | y | ||
| Q | b | y | ||
| N | b | y | ||
| P | b | y | ||
| V | b | y | ||
| Y | b | y | ||
| H | b | y | ||
| N | b | y | ||
| Q | b | y | ||
| P | b | y | ||
| L | b | y | ||
| N | b | y | ||
| P | b | y | ||
| A | b | y | ||
| P | b | y | ||
| S | b | y | ||
| R | b | y |
2. Synthetic Peptide
2.1 Synthetic (Internal Standard) Peptide Sequence
(NONE)
2.2 Peptide Properties
(NONE)
2.3 Fragment Ion Table
(NONE)
2.4 Peptide Synthesis Report
(NONE)
2.5 QqQ MS/MS
2.6 Calibration Curve of Standard
Description: NONE
Experiment Description: Cells from Human Lung caner line HCC 827 were lysed in RIPA buffer on ice. 80ugs of the cell lysate was loaded onto a SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/4 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer.
2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times
2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides
3. PTM Peptide
3.1 Native Sequence
K.RPAGSVQNPVyHNQPLNPAPSR.D (PTM: Phosphorylation)
3.2 Peptide Properties
| Start | Stop | MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|---|---|
| 1100 | 1121 | 2478.19 | 1240.1 | 827.07 | 10.84 |
3.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| R | b | y | ||
| P | b | y | ||
| A | b | y | ||
| G | b | y | ||
| S | b | y | ||
| V | b | y | ||
| Q | b | y | ||
| N | b | y | ||
| P | b | y | ||
| V | b | y | ||
| y | b | y | ||
| H | b | y | ||
| N | b | y | ||
| Q | b | y | ||
| P | b | y | ||
| L | b | y | ||
| N | b | y | ||
| P | b | y | ||
| A | b | y | ||
| P | b | y | ||
| S | b | y | ||
| R | b | y |
4. Synthetic PTM Peptide
4.1 Synthetic (Internal Standard) Peptide Sequence
(NONE)
4.2 Peptide Properties
(NONE)
4.3 Fragment Ion Table
(NONE)
4.4 Peptide Synthesis Report
(NONE)
4.5 QqQ MS/MS
4.6 Calibration Curve of Standard
Description: NONE
Experiment Description: Cells from Human Lung caner line HCC 827 were lysed in RIPA buffer on ice. 80ugs of the cell lysate was loaded onto a SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/4 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer.
4.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times
4.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides
