Information for Peptide: QTLPVIYVK from GSK3B_HUMAN

1. Biological Peptide

1.1 Native Sequence

    K.QTLPVIYVK.L

1.2 Peptide Properties

Start	Stop	MW	m/z for 2+	m/z for 3+	pI
151	159	1059.63	530.82	354.22	8.59

1.3 Fragment Ion Table

AA	b Ion Series	b Ion Mass	y Ion Series	y Ion Mass
Q	b		y
T	b		y
L	b		y
P	b		y
V	b		y
I	b		y
Y	b		y
V	b		y
K	b		y

2. Synthetic Peptide

2.1 Synthetic (Internal Standard) Peptide Sequence

QTLPVIYV(c5N1)K (Modification: Stable Isotope on V8 (U-13C5, 15N) )

2.2 Peptide Properties

MW	m/z for 2+	m/z for 3+	pI
1065.65	533.83	356.22	8.59

2.3 Fragment Ion Table

AA	b Ion Series	b Ion Mass	y Ion Series	y Ion Mass
Q	b		y
T	b		y
L	b		y
P	b		y
V	b		y
I	b		y
Y	b		y
V	b		y
K	b		y

2.4 Peptide Synthesis Report

Click to view/download synthesis report for QTLPVIYVK

2.5 QqQ MS/MS

Transition Table:

Transition	m/z of Transition	Collision Energy
y8	938.6	19.0
y7	837.55	19.0
y6	724.46	20.0
y5	627.41	24.0
y4	528.34	20.0
y3	415.26	19.0

2.6 Calibration Curve of Standard

Description: A dilution series was run from 50 fmol to 0.1 fmol. The detection limit was determined to be 5 fmol.

 

Experiment Description: Cells from colon caner line HCT 116 were lysed in 8M urea/ 100mM ammonium bicarbonate buffer on ice. The equivalent of 100,000 cells were loaded onto a SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/4 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer. Sample was analyzed concurrently with internal standard

2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times

2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides