Information for Peptide: QTALVELLK from ALBU_BOVIN
1. Biological Peptide
1.1 Native Sequence
K.QTALVELLK.H
1.2 Peptide Properties
| Start | Stop | MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|---|---|
| 549 | 557 | 1013.61 | 507.81 | 338.88 | 6.0 |
1.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| Q | b | y | ||
| T | b | y | ||
| A | b | y | ||
| L | b | y | ||
| V | b | y | ||
| E | b | y | ||
| L | b | y | ||
| L | b | y | ||
| K | b | y |
2. Synthetic Peptide
2.1 Synthetic (Internal Standard) Peptide Sequence
QTALVELL(C6N1)K (Modification: Stable Isotope label on L8 (U-13C6, 15N1) )
2.2 Peptide Properties
| MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|
| 1020.63 | 511.32 | 341.22 | 6.0 |
2.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| Q | b | y | ||
| T | b | y | ||
| A | b | y | ||
| L | b | y | ||
| V | b | y | ||
| E | b | y | ||
| L | b | y | ||
| L | b | y | ||
| K | b | y |
2.4 Peptide Synthesis Report
Click to view/download synthesis report for QTALVELLK
2.5 QqQ MS/MS
Transition Table:
| Transition | m/z of Transition | Collision Energy |
|---|---|---|
| y5 | 608.409103 | 21.0 |
| y6 | 721.493167 | 21.0 |
| y7 | 792.530281 | 21.0 |
2.6 Calibration Curve of Standard
Description: NONE
Experiment Description: 10fmol of BSA standard was analysed on the TSQ. Sample was analyzed concurrently with synthetic internal standards
2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times
2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides
