Information for Peptide: QLAEEYLYR from MMP9_HUMAN
1. Biological Peptide
1.1 Native Sequence
R.QLAEEYLYR.Y
1.2 Peptide Properties
| Start | Stop | MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|---|---|
| 43 | 51 | 1183.59 | 592.8 | 395.54 | 4.53 |
1.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| Q | b | y | ||
| L | b | y | ||
| A | b | y | ||
| E | b | y | ||
| E | b | y | ||
| Y | b | y | ||
| L | b | y | ||
| Y | b | y | ||
| R | b | y |
2. Synthetic Peptide
2.1 Synthetic (Internal Standard) Peptide Sequence
QLAEEYL(C6N1)YR (Modification: Stable isotope on L7 (U-13C, 15N) )
2.2 Peptide Properties
| MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|
| 1190.6 | 596.31 | 397.88 | 4.53 |
2.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| Q | b | y | ||
| L | b | y | ||
| A | b | y | ||
| E | b | y | ||
| E | b | y | ||
| Y | b | y | ||
| L | b | y | ||
| Y | b | y | ||
| R | b | y |
2.4 Peptide Synthesis Report
Click to view/download synthesis report for QLAEEYLYR
2.5 QqQ MS/MS
2.6 Calibration Curve of Standard
Description: Internal standard was diluted from 50 fmol to 100 amol injection concentrations and was found to be sensitive down to 0.1 fmol
Experiment Description: Control urine was processed via ultrafiltration according to Moffitt Proteomics protocol and subjected to in-solution digestion with trypsin, following thermal denaturation, reduction, and alkylation. The sample was then analyzed on a Thermo Scientific TSQ mass spectrometer. Sample was analyzed concurrently with internal standard
2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times
2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides
