Information for Peptide: NSDEINIPR from FACD2_HUMAN
1. Biological Peptide
1.1 Native Sequence
K.NSDEINIPR.L
1.2 Peptide Properties
| Start | Stop | MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|---|---|
| 166 | 174 | 1056.52 | 529.27 | 353.18 | 4.37 |
1.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| N | b | y | ||
| S | b | y | ||
| D | b | y | ||
| E | b | y | ||
| I | b | y | ||
| N | b | y | ||
| I | b | y | ||
| P | b | y | ||
| R | b | y |
2. Synthetic Peptide
2.1 Synthetic (Internal Standard) Peptide Sequence
NSDEINIP(C5N1)R (Modification: Stable Isotope on P8 (u-13C5, 15N) )
2.2 Peptide Properties
| MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|
| 1062.53 | 532.27 | 355.19 | 4.37 |
2.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| N | b | y | ||
| S | b | y | ||
| D | b | y | ||
| E | b | y | ||
| I | b | y | ||
| N | b | y | ||
| I | b | y | ||
| P | b | y | ||
| R | b | y |
2.4 Peptide Synthesis Report
Click to view/download synthesis report for NSDEINIPR
2.5 QqQ MS/MS
Transition Table:
| Transition | m/z of Transition | Collision Energy |
|---|---|---|
| y4 | 505.3 | 18.0 |
| y5 | 618.38 | 23.0 |
| y6 | 747.43 | 22.0 |
| y7 | 862.45 | 26.0 |
2.6 Calibration Curve of Standard
Description: A dilution series was done on the internal standards with concentrations ranging from 50 fmol to 0.1 fmol. Peptide sensitivity was reliable down to 0.1 fmol.
Experiment Description: Cells from caner line 8226 were lysed in RIPA buffer on ice. The equivalent of 100,000 cells were loaded onto a SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/4 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer.
2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times
2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides
