Information for Peptide: NQVALNPQNTVFDAK from HSP71_HUMAN
1. Biological Peptide
1.1 Native Sequence
K.NQVALNPQNTVFDAK.R
1.2 Peptide Properties
| Start | Stop | MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|---|---|
| 57 | 71 | 1657.84 | 829.93 | 553.62 | 5.84 |
1.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| N | b | y | ||
| Q | b | y | ||
| V | b | y | ||
| A | b | y | ||
| L | b | y | ||
| N | b | y | ||
| P | b | y | ||
| Q | b | y | ||
| N | b | y | ||
| T | b | y | ||
| V | b | y | ||
| F | b | y | ||
| D | b | y | ||
| A | b | y | ||
| K | b | y |
2. Synthetic Peptide
2.1 Synthetic (Internal Standard) Peptide Sequence
NQVALNPQNTV(c5n1)FDAK (Modification: STable isotope label on V11 (U-C13, 15N) )
2.2 Peptide Properties
| MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|
| 1663.86 | 832.94 | 555.63 | 5.84 |
2.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| N | b | y | ||
| Q | b | y | ||
| V | b | y | ||
| A | b | y | ||
| L | b | y | ||
| N | b | y | ||
| P | b | y | ||
| Q | b | y | ||
| N | b | y | ||
| T | b | y | ||
| V | b | y | ||
| F | b | y | ||
| D | b | y | ||
| A | b | y | ||
| K | b | y |
2.4 Peptide Synthesis Report
Click to view/download synthesis report for NQVALNPQNTVFDAK
2.5 QqQ MS/MS
Transition Table:
| Transition | m/z of Transition | Collision Energy |
|---|---|---|
| y3 | 333.17745 | 33.0 |
| y4 | 480.24587 | 33.0 |
| y5 | 585.31428 | 33.0 |
| y6 | 686.36169 | 33.0 |
| y7 | 800.40489 | 33.0 |
| y8 | 928.46346 | 33.0 |
2.6 Calibration Curve of Standard
Description: NONE
Experiment Description: Cells from AML line U937 were lysed in 8M urea/ 100mM ammonium bicarbonate buffer on ice. The equivalent of 100,000 cells were loaded onto a SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/4 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer.
2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times
2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides
