Information for Peptide: NEVGVLR from BRAF_HUMAN
1. Biological Peptide
1.1 Native Sequence
K.NEVGVLR.K
1.2 Peptide Properties
| Start | Stop | MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|---|---|
| 500 | 506 | 785.44 | 393.73 | 262.82 | 6.0 |
1.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| N | b | y | ||
| E | b | y | ||
| V | b | y | ||
| G | b | y | ||
| V | b | y | ||
| L | b | y | ||
| R | b | y |
2. Synthetic Peptide
2.1 Synthetic (Internal Standard) Peptide Sequence
NEVGVL(C6N1)R (Modification: Stable Isotope label on L6 (13C, 15N) )
2.2 Peptide Properties
| MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|
| 792.46 | 397.24 | 265.16 | 6.0 |
2.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| N | b | y | ||
| E | b | y | ||
| V | b | y | ||
| G | b | y | ||
| V | b | y | ||
| L | b | y | ||
| R | b | y |
2.4 Peptide Synthesis Report
Click to view/download synthesis report for NEVGVLR
2.5 QqQ MS/MS
Transition Table:
| Transition | m/z of Transition | Collision Energy |
|---|---|---|
| y6 | 679.40449 | 14.0 |
| y5 | 550.3619 | 16.0 |
| y4 | 451.29349 | 15.0 |
| y3 | 394.27202 | 18.0 |
2.6 Calibration Curve of Standard
Description: A dilution series was done on the internal standard peptide from 50 Fmol to 0.1 fmol. Peptide detection was possible to 0.1 fmol.
Experiment Description: Cells from the Melanoma cancer cell line were lysed in RIPA buffer. The equivalent of 200,000 cells were loaded onto an SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/6 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer.
2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times
2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides
