Information for Peptide: LVNEVTEFAK from ALBU_HUMAN
1. Biological Peptide
1.1 Native Sequence
K.LVNEVTEFAK.T
1.2 Peptide Properties
| Start | Stop | MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|---|---|
| 66 | 75 | 1148.61 | 575.31 | 383.88 | 4.53 |
1.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| L | b | y | ||
| V | b | y | ||
| N | b | y | ||
| E | b | y | ||
| V | b | y | ||
| T | b | y | ||
| E | b | y | ||
| F | b | y | ||
| A | b | y | ||
| K | b | y |
2. Synthetic Peptide
2.1 Synthetic (Internal Standard) Peptide Sequence
LVNDVTEFAK (Modification: E4 to D )
2.2 Peptide Properties
| MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|
| 1134.59 | 568.3 | 379.21 | 4.37 |
2.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| L | b | y | ||
| V | b | y | ||
| N | b | y | ||
| D | b | y | ||
| V | b | y | ||
| T | b | y | ||
| E | b | y | ||
| F | b | y | ||
| A | b | y | ||
| K | b | y |
2.4 Peptide Synthesis Report
Click to view/download synthesis report for LVNEVTEFAK
2.5 QqQ MS/MS
Transition Table:
| Transition | m/z of Transition | Collision Energy |
|---|---|---|
| y4 | 494.26 | 23.0 |
| y5 | 595.31 | 23.0 |
| y7 | 809.4 | 23.0 |
| y8 | 923.45 | 23.0 |
2.6 Calibration Curve of Standard
Description: NONE
Experiment Description: Serum sample digest from healthy adult venous blood draw. Sample denatured in Urea, reduced and alkylated (20mM TCEP and 200mM IAA) and digested overnight with trypsin. Sample was diluted in 2% ACN with 0.1% formic acid and a total of 0.1 nanoliters of serum was injected onto column.
2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times
2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides
