Information for Peptide: LVDQNIFSFYLSR from CATD_HUMAN
1. Biological Peptide
1.1 Native Sequence
K.LVDQNIFSFYLSR.D
1.2 Peptide Properties
| Start | Stop | MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|---|---|
| 223 | 235 | 1600.82 | 801.42 | 534.62 | 5.84 |
1.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| L | b | y | ||
| V | b | y | ||
| D | b | y | ||
| Q | b | y | ||
| N | b | y | ||
| I | b | y | ||
| F | b | y | ||
| S | b | y | ||
| F | b | y | ||
| Y | b | y | ||
| L | b | y | ||
| S | b | y | ||
| R | b | y |
2. Synthetic Peptide
2.1 Synthetic (Internal Standard) Peptide Sequence
LVDQNIFSFYL(C6N1)SR (Modification: Stable isotope label on L11 (U-13C, 15N) )
2.2 Peptide Properties
| MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|
| 1607.84 | 804.93 | 536.96 | 5.84 |
2.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| L | b | y | ||
| V | b | y | ||
| D | b | y | ||
| Q | b | y | ||
| N | b | y | ||
| I | b | y | ||
| F | b | y | ||
| S | b | y | ||
| F | b | y | ||
| Y | b | y | ||
| L | b | y | ||
| S | b | y | ||
| R | b | y |
2.4 Peptide Synthesis Report
Click to view/download synthesis report for LVDQNIFSFYLSR
2.5 QqQ MS/MS
Transition Table:
| Transition | m/z of Transition | Collision Energy |
|---|---|---|
| y8 | 1039.55188 | 29.0 |
| y7 | 926.46782 | 28.0 |
| y6 | 779.39941 | 28.0 |
2.6 Calibration Curve of Standard
Description: NONE
Experiment Description: Control urine was processed via ultrafiltration according to Moffitt Proteomics protocol and subjected to in-solution digestion with trypsin, following thermal denaturation, reduction, and alkylation.
2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times
2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides
