Information for Peptide: LQAEAQQLR from CDC37_HUMAN
1. Biological Peptide
1.1 Native Sequence
R.LQAEAQQLR.K
1.2 Peptide Properties
| Start | Stop | MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|---|---|
| 84 | 92 | 1055.57 | 528.79 | 352.87 | 6.0 |
1.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| L | b | y | ||
| Q | b | y | ||
| A | b | y | ||
| E | b | y | ||
| A | b | y | ||
| Q | b | y | ||
| Q | b | y | ||
| L | b | y | ||
| R | b | y |
2. Synthetic Peptide
2.1 Synthetic (Internal Standard) Peptide Sequence
LQAEAQQL(c6N1)R (Modification: Stable Isotope on L8 (U-13C6, 15N) )
2.2 Peptide Properties
| MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|
| 1062.59 | 532.3 | 355.2 | 6.0 |
2.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| L | b | y | ||
| Q | b | y | ||
| A | b | y | ||
| E | b | y | ||
| A | b | y | ||
| Q | b | y | ||
| Q | b | y | ||
| L | b | y | ||
| R | b | y |
2.4 Peptide Synthesis Report
Click to view/download synthesis report for LQAEAQQLR
2.5 QqQ MS/MS
Transition Table:
| Transition | m/z of Transition | Collision Energy |
|---|---|---|
| y4 | 551.32 | 21.0 |
| y5 | 622.36 | 21.0 |
| y6 | 751.4 | 21.0 |
| y7 | 822.44 | 21.0 |
2.6 Calibration Curve of Standard
Description: NONE
Experiment Description: Cells from AML line U937 were lysed in 8M urea/ 100mM ammonium bicarbonate buffer on ice. The lysate digested insolution with trypsin was performed after reduction and alkylation with TCEP and IAA.
2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times
2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides
