Information for Peptide: LPEPLEDGLK from TCF7_HUMAN

1. Biological Peptide

1.1 Native Sequence

    K.LPEPLEDGLK.A

1.2 Peptide Properties

Start	Stop	MW	m/z for 2+	m/z for 3+	pI
99	108	1109.6	555.81	370.87	4.14

1.3 Fragment Ion Table

AA	b Ion Series	b Ion Mass	y Ion Series	y Ion Mass
L	b		y
P	b		y
E	b		y
P	b		y
L	b		y
E	b		y
D	b		y
G	b		y
L	b		y
K	b		y

2. Synthetic Peptide

2.1 Synthetic (Internal Standard) Peptide Sequence

LPEPLEDGL(C6N1)K (Modification: Stable Isotope on L9 (U-13C6, 15N) )

2.2 Peptide Properties

MW	m/z for 2+	m/z for 3+	pI
1116.61	559.31	373.21	4.14

2.3 Fragment Ion Table

AA	b Ion Series	b Ion Mass	y Ion Series	y Ion Mass
L	b		y
P	b		y
E	b		y
P	b		y
L	b		y
E	b		y
D	b		y
G	b		y
L	b		y
K	b		y

2.4 Peptide Synthesis Report

Click to view/download synthesis report for LPEPLEDGLK

2.5 QqQ MS/MS

Transition Table:

Transition	m/z of Transition	Collision Energy
y3	324.22	22.0
y4	439.25	22.0
y5	568.29	22.0
y8	907.47	22.0

2.6 Calibration Curve of Standard

Description: A dilution curve was done on the internal standard peptide from 50 fmol to 0.1 fmol. Senisitivty was determined down to 5 fmol.

 

Experiment Description: Cells from colon caner line HCT 116 were lysed in 8M urea/ 100mM ammonium bicarbonate buffer on ice. The equivalent of 100,000 cells were loaded onto a SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/4 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer. Internal standard has been synthesized and is awaitng analysis with sample

2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times

2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides