Information for Peptide: LLLETAAPR from AXN1_HUMAN

1. Biological Peptide

1.1 Native Sequence

    K.LLLETAAPR.V

1.2 Peptide Properties

Start	Stop	MW	m/z for 2+	m/z for 3+	pI
271	279	982.58	492.3	328.53	6.0

1.3 Fragment Ion Table

AA	b Ion Series	b Ion Mass	y Ion Series	y Ion Mass
L	b		y
L	b		y
L	b		y
E	b		y
T	b		y
A	b		y
A	b		y
P	b		y
R	b		y

2. Synthetic Peptide

2.1 Synthetic (Internal Standard) Peptide Sequence

LLLETAAP(c5N1)R (Modification: Stable Isotope on P8 (U-13C5, 15N1) )

2.2 Peptide Properties

MW	m/z for 2+	m/z for 3+	pI
988.59	495.31	330.54	6.0

2.3 Fragment Ion Table

AA	b Ion Series	b Ion Mass	y Ion Series	y Ion Mass
L	b		y
L	b		y
L	b		y
E	b		y
T	b		y
A	b		y
A	b		y
P	b		y
R	b		y

2.4 Peptide Synthesis Report

Click to view/download synthesis report for LLLETAAPR

2.5 QqQ MS/MS

Transition Table:

Transition	m/z of Transition	Collision Energy
y8	876.5	20.0
y7	763.42	20.0
y6	650.34	20.0
y5	521.29	20.0
y4	420.25	20.0
y3	349.21	20.0

2.6 Calibration Curve of Standard

Description: Internal standard was diluted from 50 fmol to 500 amol injection concentrations and was found to be sensitive down to 0.1 fmol

 

Experiment Description: Cells from colon caner line HCT 116 were lysed in 8M urea/ 100mM ammonium bicarbonate buffer on ice. The equivalent of 100,000 cells were loaded onto a SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/4 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer. Internal standard has been synthesized and is awaiting analysis concurrently with sample.

2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times

2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides