Information for Peptide: LFWTDTGINPR from EGF_HUMAN
1. Biological Peptide
1.1 Native Sequence
R.LFWTDTGINPR.I
1.2 Peptide Properties
| Start | Stop | MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|---|---|
| 612 | 622 | 1318.67 | 660.34 | 440.56 | 5.84 |
1.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| L | b | y | ||
| F | b | y | ||
| W | b | y | ||
| T | b | y | ||
| D | b | y | ||
| T | b | y | ||
| G | b | y | ||
| I | b | y | ||
| N | b | y | ||
| P | b | y | ||
| R | b | y |
2. Synthetic Peptide
2.1 Synthetic (Internal Standard) Peptide Sequence
LFWTDTGINP(C5N1)R (Modification: Stable isotope on P10 (U-13C, 15N) )
2.2 Peptide Properties
| MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|
| 1324.68 | 663.35 | 442.57 | 5.84 |
2.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| L | b | y | ||
| F | b | y | ||
| W | b | y | ||
| T | b | y | ||
| D | b | y | ||
| T | b | y | ||
| G | b | y | ||
| I | b | y | ||
| N | b | y | ||
| P | b | y | ||
| R | b | y |
2.4 Peptide Synthesis Report
Click to view/download synthesis report for LFWTDTGINPR
2.5 QqQ MS/MS
Transition Table:
| Transition | m/z of Transition | Collision Energy |
|---|---|---|
| y3 | 392.2152 | 25.8 |
| y5 | 562.3207 | 24.0 |
| y7 | 778.3953 | 25.0 |
| y8 | 879.443 | 25.8 |
| y9 | 1065.5223 | 23.0 |
2.6 Calibration Curve of Standard
Description: A dilution series was done on the internal standard peptide from 50 Fmol to 0.1 fmol. Peptide detection was possible to 0.1 fmol.
Experiment Description: Control urine was processed via ultrafiltration according to Moffitt Proteomics protocol and subjected to in-solution digestion with trypsin, following thermal denaturation, reduction, and alkylation.
2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times
2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides
