Information for Peptide: LFLIDFGLAK from KC1A_HUMAN
1. Biological Peptide
1.1 Native Sequence
K.LFLIDFGLAK.K
1.2 Peptide Properties
| Start | Stop | MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|---|---|
| 153 | 162 | 1135.66 | 568.84 | 379.56 | 5.84 |
1.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| L | b | y | ||
| F | b | y | ||
| L | b | y | ||
| I | b | y | ||
| D | b | y | ||
| F | b | y | ||
| G | b | y | ||
| L | b | y | ||
| A | b | y | ||
| K | b | y |
2. Synthetic Peptide
2.1 Synthetic (Internal Standard) Peptide Sequence
LFLIDFGLGK (Modification: A9 to G )
2.2 Peptide Properties
| MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|
| 1121.65 | 561.83 | 374.89 | 5.84 |
2.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| L | b | y | ||
| F | b | y | ||
| L | b | y | ||
| I | b | y | ||
| D | b | y | ||
| F | b | y | ||
| G | b | y | ||
| L | b | y | ||
| G | b | y | ||
| K | b | y |
2.4 Peptide Synthesis Report
Click to view/download synthesis report for LFLIDFGLAK
2.5 QqQ MS/MS
Transition Table:
| Transition | m/z of Transition | Collision Energy |
|---|---|---|
| y4 | 374.24 | 22.0 |
| y5 | 521.31 | 22.0 |
| y6 | 636.34 | 22.0 |
| y7 | 749.42 | 22.0 |
| y9 | 1009.57 | 22.0 |
2.6 Calibration Curve of Standard
Description: NONE
Experiment Description: Cells from colon caner line HCT 116 were lysed in 8M urea/ 100mM ammonium bicarbonate buffer on ice. The equivalent of 100,000 cells were loaded onto a SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/4 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer. Internal standard synthesized and awaiting analysis with sample.
2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times
2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides
