Information for Peptide: ITVVDALHEIPVK from IBP7_HUMAN
1. Biological Peptide
1.1 Native Sequence
K.ITVVDALHEIPVK.K
1.2 Peptide Properties
| Start | Stop | MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|---|---|
| 263 | 275 | 1432.83 | 717.42 | 478.62 | 5.32 |
1.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| I | b | y | ||
| T | b | y | ||
| V | b | y | ||
| V | b | y | ||
| D | b | y | ||
| A | b | y | ||
| L | b | y | ||
| H | b | y | ||
| E | b | y | ||
| I | b | y | ||
| P | b | y | ||
| V | b | y | ||
| K | b | y |
2. Synthetic Peptide
2.1 Synthetic (Internal Standard) Peptide Sequence
ITVVDALHEIP(C5N1)VK (Modification: Stable Isotope on P11 (U-13C5, 15N) )
2.2 Peptide Properties
| MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|
| 1438.84 | 720.43 | 480.62 | 5.32 |
2.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| I | b | y | ||
| T | b | y | ||
| V | b | y | ||
| V | b | y | ||
| D | b | y | ||
| A | b | y | ||
| L | b | y | ||
| H | b | y | ||
| E | b | y | ||
| I | b | y | ||
| P | b | y | ||
| V | b | y | ||
| K | b | y |
2.4 Peptide Synthesis Report
Click to view/download synthesis report for ITVVDALHEIPVK
2.5 QqQ MS/MS
Transition Table:
| Transition | m/z of Transition | Collision Energy |
|---|---|---|
| y3 | 349.2346 | 27.7 |
| y4 | 462.3186 | 27.7 |
| y5 | 591.3612 | 27.7 |
| y8 | 912.5413 | 27.7 |
2.6 Calibration Curve of Standard
Description: NONE
Experiment Description: Control urine was processed via ultrafiltration according to Moffitt Proteomics protocol and subjected to in-solution digestion with trypsin, following thermal denaturation, reduction, and alkylation.
2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times
2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides
