Information for Peptide: HSQFLGYPITLYLEK from H90B2_HUMAN
1. Biological Peptide
1.1 Native Sequence
K.HSQFLGYPITLYLEK.E
1.2 Peptide Properties
| Start | Stop | MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|---|---|
| 127 | 141 | 1807.95 | 904.98 | 603.66 | 6.75 |
1.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| H | b | y | ||
| S | b | y | ||
| Q | b | y | ||
| F | b | y | ||
| L | b | y | ||
| G | b | y | ||
| Y | b | y | ||
| P | b | y | ||
| I | b | y | ||
| T | b | y | ||
| L | b | y | ||
| Y | b | y | ||
| L | b | y | ||
| E | b | y | ||
| K | b | y |
2. Synthetic Peptide
2.1 Synthetic (Internal Standard) Peptide Sequence
HSQFLGYPITLYL(C6N1)EK (Modification: Stable Isotope on L13 (U-13C6, 15N) )
2.2 Peptide Properties
| MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|
| 1814.97 | 908.49 | 606.0 | 6.75 |
2.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| H | b | y | ||
| S | b | y | ||
| Q | b | y | ||
| F | b | y | ||
| L | b | y | ||
| G | b | y | ||
| Y | b | y | ||
| P | b | y | ||
| I | b | y | ||
| T | b | y | ||
| L | b | y | ||
| Y | b | y | ||
| L | b | y | ||
| E | b | y | ||
| K | b | y |
2.4 Peptide Synthesis Report
Click to view/download synthesis report for HSQFLGYPITLYLEK
2.5 QqQ MS/MS
Transition Table:
| Transition | m/z of Transition | Collision Energy |
|---|---|---|
| y3 | 396.26 | 34.0 |
| y4 | 559.32 | 34.0 |
| y5 | 672.4 | 34.0 |
| y6 | 773.45 | 34.0 |
| y7 | 886.51919 | 34.0 |
| y8 | 983.57195 | 34.0 |
| y9 | 1146.63528 | 34.0 |
| y10 | 1203.65674 | 34.0 |
| y11 | 1316.76 | 34.0 |
| y12 | 1463.83 | 34.0 |
2.6 Calibration Curve of Standard
Description: NONE
Experiment Description: Cells from AML line U937 were lysed in 8M urea/ 100mM ammonium bicarbonate buffer on ice. The equivalent of 100,000 cells were loaded onto a SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/4 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer.
2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times
2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides
