Information for Peptide: GYASPDLSK from RAF1_HUMAN
1. Biological Peptide
1.1 Native Sequence
R.GYASPDLSK.L
1.2 Peptide Properties
| Start | Stop | MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|---|---|
| 564 | 572 | 936.46 | 469.24 | 313.16 | 5.83 |
1.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| G | b | y | ||
| Y | b | y | ||
| A | b | y | ||
| S | b | y | ||
| P | b | y | ||
| D | b | y | ||
| L | b | y | ||
| S | b | y | ||
| K | b | y |
2. Synthetic Peptide
2.1 Synthetic (Internal Standard) Peptide Sequence
(NONE)
2.2 Peptide Properties
(NONE)
2.3 Fragment Ion Table
(NONE)
2.4 Peptide Synthesis Report
(NONE)
2.5 QqQ MS/MS
2.6 Calibration Curve of Standard
Description: NONE
Experiment Description: Cells from Melanoma cell lines were lysed in 8M urea/ 100mM ammonium bicarbonate buffer on ice. The equivalent of 200,000 cells were loaded onto a SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/6 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer.
2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times
2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides
