Information for Peptide: GVTIPSQR from PTEN_HUMAN

1. Biological Peptide

1.1 Native Sequence

    K.GVTIPSQR.R

1.2 Peptide Properties

Start	Stop	MW	m/z for 2+	m/z for 3+	pI
165	172	856.48	429.25	286.5	9.75

1.3 Fragment Ion Table

AA	b Ion Series	b Ion Mass	y Ion Series	y Ion Mass
G	b		y
V	b		y
T	b		y
I	b		y
P	b		y
S	b		y
Q	b		y
R	b		y

2. Synthetic Peptide

2.1 Synthetic (Internal Standard) Peptide Sequence

GVTIPSNR (Modification: Amino acid switch from Q7 to N )

2.2 Peptide Properties

MW	m/z for 2+	m/z for 3+	pI
842.46	422.24	281.83	9.75

2.3 Fragment Ion Table

AA	b Ion Series	b Ion Mass	y Ion Series	y Ion Mass
G	b		y
V	b		y
T	b		y
I	b		y
P	b		y
S	b		y
N	b		y
R	b		y

2.4 Peptide Synthesis Report

Click to view/download synthesis report for GVTIPSQR

2.5 QqQ MS/MS

Transition Table:

Transition	m/z of Transition	Collision Energy
y7	786.44742	10.0
y6	687.37901	17.0
y5	586.33133	17.0
y4	473.24726	14.0
y3	376.1945	26.0
b4	371.22949	10.0
b3	258.14543	15.0

2.6 Calibration Curve of Standard

Description: A dilution series was done on the internal standard peptide from 50 Fmol to 0.1 fmol. Peptide detection was possible to 0.1 fmol.

 

Experiment Description: Cells from Melanoma cell lines were lysed in RIPA buffer. The equivalent of 200,000 cells were loaded onto an SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/6 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer.

2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times

2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides