Information for Peptide: GTLDPVEK from HSP7C_HUMAN
1. Biological Peptide
1.1 Native Sequence
R.GTLDPVEK.A
1.2 Peptide Properties
| Start | Stop | MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|---|---|
| 312 | 319 | 857.45 | 429.73 | 286.82 | 4.37 |
1.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| G | b | y | ||
| T | b | y | ||
| L | b | y | ||
| D | b | y | ||
| P | b | y | ||
| V | b | y | ||
| E | b | y | ||
| K | b | y |
2. Synthetic Peptide
2.1 Synthetic (Internal Standard) Peptide Sequence
GTLDPV(C5N1)EK (Modification: Stable Isotope on V6 (U-13C, 15N) )
2.2 Peptide Properties
| MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|
| 863.46 | 432.74 | 288.83 | 4.37 |
2.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| G | b | y | ||
| T | b | y | ||
| L | b | y | ||
| D | b | y | ||
| P | b | y | ||
| V | b | y | ||
| E | b | y | ||
| K | b | y |
2.4 Peptide Synthesis Report
Click to view/download synthesis report for GTLDPVEK
2.5 QqQ MS/MS
Transition Table:
| Transition | m/z of Transition | Collision Energy |
|---|---|---|
| y4 | 478.27717 | 17.0 |
| y5 | 593.30411 | 17.0 |
| y6 | 706.38817 | 17.0 |
2.6 Calibration Curve of Standard
Description: NONE
Experiment Description: Cells from HCT116 were lysed in 8M urea/ 100mM ammonium bicarbonate buffer on ice. The equivalent of 100,000 cells were loaded onto a SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/4 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer.
2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times
2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides
