Information for Peptide: GPSWDPFR from HSPB1_HUMAN

1. Biological Peptide

1.1 Native Sequence

    R.GPSWDPFR.D

1.2 Peptide Properties

Start	Stop	MW	m/z for 2+	m/z for 3+	pI
13	20	960.45	481.23	321.16	5.84

1.3 Fragment Ion Table

AA	b Ion Series	b Ion Mass	y Ion Series	y Ion Mass
G	b		y
P	b		y
S	b		y
W	b		y
D	b		y
P	b		y
F	b		y
R	b		y

2. Synthetic Peptide

2.1 Synthetic (Internal Standard) Peptide Sequence

(NONE)

2.2 Peptide Properties

(NONE)

2.3 Fragment Ion Table

(NONE)

2.4 Peptide Synthesis Report

(NONE)

2.5 QqQ MS/MS

2.6 Calibration Curve of Standard

Description: NONE

 

Experiment Description: Cells from AML line U937 were lysed in 8M urea/ 100mM ammonium bicarbonate buffer on ice. The lysate was digested insolution with trypsin after reduction and alkylation with TCEP and IAA.

2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times

2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides