Information for Peptide: GPSVFPLAPSSK from IGHG1_HUMAN

1. Biological Peptide

1.1 Native Sequence

    K.GPSVFPLAPSSK.S

1.2 Peptide Properties

Start	Stop	MW	m/z for 2+	m/z for 3+	pI
5	16	1185.64	593.83	396.22	8.75

1.3 Fragment Ion Table

AA	b Ion Series	b Ion Mass	y Ion Series	y Ion Mass
G	b		y
P	b		y
S	b		y
V	b		y
F	b		y
P	b		y
L	b		y
A	b		y
P	b		y
S	b		y
S	b		y
K	b		y

2. Synthetic Peptide

2.1 Synthetic (Internal Standard) Peptide Sequence

GPSVFPLAP(C5N1)SSK (Modification: Stable Isotope on P9 (U-13C5, 15N) )

2.2 Peptide Properties

MW	m/z for 2+	m/z for 3+	pI
1191.65	596.83	398.23	8.75

2.3 Fragment Ion Table

AA	b Ion Series	b Ion Mass	y Ion Series	y Ion Mass
G	b		y
P	b		y
S	b		y
V	b		y
F	b		y
P	b		y
L	b		y
A	b		y
P	b		y
S	b		y
S	b		y
K	b		y

2.4 Peptide Synthesis Report

Click to view/download synthesis report for GPSVFPLAPSSK

2.5 QqQ MS/MS

Transition Table:

Transition	m/z of Transition	Collision Energy
y8	852.472	24.0
y9	951.54	24.0
y10	1038.573	24.0

2.6 Calibration Curve of Standard

Description: Internal standard GPSVFPLAPSSK was diluted from 50 fmol to 500 amol injection concentrations and was found to be sensitive down to 5 fmol

 

Experiment Description: Serum sample digest from healthy adult venous blood draw. Sample denatured in Urea, reduced and alkylated (20mM TCEP and 200mM IAA) and digested overnight with trypsin. Sample was diluted in 2% ACN with 0.1% formic acid and a total of 0.1 nanoliters of serum was injected onto column.

2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times

2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides