Information for Peptide: GLGPSPAGDGPSGSGK from BAD_HUMAN
1. Biological Peptide
1.1 Native Sequence
R.GLGPSPAGDGPSGSGK.H
1.2 Peptide Properties
| Start | Stop | MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|---|---|
| 21 | 36 | 1339.64 | 670.83 | 447.55 | 5.84 |
1.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| G | b | y | ||
| L | b | y | ||
| G | b | y | ||
| P | b | y | ||
| S | b | y | ||
| P | b | y | ||
| A | b | y | ||
| G | b | y | ||
| D | b | y | ||
| G | b | y | ||
| P | b | y | ||
| S | b | y | ||
| G | b | y | ||
| S | b | y | ||
| G | b | y | ||
| K | b | y |
2. Synthetic Peptide
2.1 Synthetic (Internal Standard) Peptide Sequence
GL(c6n1)GPSPAGDGPSGSGK (Modification: Stable Isotope on L2 (U-13C6, 15N) )
2.2 Peptide Properties
| MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|
| 1346.65 | 674.33 | 449.89 | 5.84 |
2.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| G | b | y | ||
| L | b | y | ||
| G | b | y | ||
| P | b | y | ||
| S | b | y | ||
| P | b | y | ||
| A | b | y | ||
| G | b | y | ||
| D | b | y | ||
| G | b | y | ||
| P | b | y | ||
| S | b | y | ||
| G | b | y | ||
| S | b | y | ||
| G | b | y | ||
| K | b | y |
2.4 Peptide Synthesis Report
Click to view/download synthesis report for GLGPSPAGDGPSGSGK
2.5 QqQ MS/MS
Transition Table:
| Transition | m/z of Transition | Collision Energy |
|---|---|---|
| y13 | 1113.52 | 25.0 |
| y12 | 1016.46 | 29.0 |
| y11 | 929.43 | 30.0 |
| y10 | 832.38 | 39.0 |
| y9 | 761.34 | 29.0 |
2.6 Calibration Curve of Standard
Description: A dilution series was done on the internal standard peptide from 50 Fmol to 0.1 fmol. Peptide detection was possible to 0.1 fmol.
Experiment Description: Cells from line 8226 were lysed in RIPA buffer. The equivalent of 50,000 cells were loaded onto an SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/4 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer. Sample was analyzed concurrently with internal standard
2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times
2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides
