Information for Peptide: GGEIQPVSVK from CH10_HUMAN

1. Biological Peptide

1.1 Native Sequence

    K.GGEIQPVSVK.V

1.2 Peptide Properties

Start	Stop	MW	m/z for 2+	m/z for 3+	pI
57	66	1012.56	507.29	338.53	6.0

1.3 Fragment Ion Table

AA	b Ion Series	b Ion Mass	y Ion Series	y Ion Mass
G	b		y
G	b		y
E	b		y
I	b		y
Q	b		y
P	b		y
V	b		y
S	b		y
V	b		y
K	b		y

2. Synthetic Peptide

2.1 Synthetic (Internal Standard) Peptide Sequence

GGEIQPVSV(C5N1)K (Modification: Stable isotope on V9 (U-13C, 15N) )

2.2 Peptide Properties

MW	m/z for 2+	m/z for 3+	pI
1018.57	510.29	340.53	6.0

2.3 Fragment Ion Table

AA	b Ion Series	b Ion Mass	y Ion Series	y Ion Mass
G	b		y
G	b		y
E	b		y
I	b		y
Q	b		y
P	b		y
V	b		y
S	b		y
V	b		y
K	b		y

2.4 Peptide Synthesis Report

Click to view/download synthesis report for GGEIQPVSVK

2.5 QqQ MS/MS

Transition Table:

Transition	m/z of Transition	Collision Energy
b4	357.17745	16.0
b5	485.23603	15.0
b7	681.35721	14.0
y3	339.21384	24.0
y4	438.28225	26.0
y5	535.33502	18.0
y6	663.39359	16.0
y7	776.47766	19.0
y8	905.52025	17.0
y9	962.54171	47.0

2.6 Calibration Curve of Standard

Description: NONE

 

Experiment Description: Cells from line 8226 were lysed in 8M urea/100mM ammonium bicarbonate and then were subjected to in-solution digestion with trypsin, following reduction and alkylation. The digest was then diluted down to the equivalent of 500 cells and injected concurrently with internal standards into a Thermo Scientific TSQ mass spectrometer.

2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times

2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides