Information for Peptide: GDLGIEIPAEK from KPYM_HUMAN
1. Biological Peptide
1.1 Native Sequence
R.GDLGIEIPAEK.V
1.2 Peptide Properties
| Start | Stop | MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|---|---|
| 295 | 305 | 1140.6 | 571.31 | 381.21 | 4.14 |
1.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| G | b | y | ||
| D | b | y | ||
| L | b | y | ||
| G | b | y | ||
| I | b | y | ||
| E | b | y | ||
| I | b | y | ||
| P | b | y | ||
| A | b | y | ||
| E | b | y | ||
| K | b | y |
2. Synthetic Peptide
2.1 Synthetic (Internal Standard) Peptide Sequence
GDLGIEIP(C5N1)AEK (Modification: Stable isotope on P8 (U-13C, 15N) )
2.2 Peptide Properties
| MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|
| 1146.62 | 574.32 | 383.21 | 4.14 |
2.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| G | b | y | ||
| D | b | y | ||
| L | b | y | ||
| G | b | y | ||
| I | b | y | ||
| E | b | y | ||
| I | b | y | ||
| P | b | y | ||
| A | b | y | ||
| E | b | y | ||
| K | b | y |
2.4 Peptide Synthesis Report
Click to view/download synthesis report for GDLGIEIPAEK
2.5 QqQ MS/MS
Transition Table:
| Transition | m/z of Transition | Collision Energy |
|---|---|---|
| b4 | 343.1618 | 21.0 |
| b5 | 456.24587 | 16.0 |
| b6 | 585.28846 | 18.0 |
| b7 | 698.37252 | 15.0 |
| y3 | 347.1931 | 35.0 |
| y4 | 450.24587 | 26.0 |
| y5 | 563.32993 | 18.0 |
| y6 | 692.37252 | 17.0 |
| y7 | 805.45659 | 21.0 |
| y8 | 862.47805 | 19.0 |
2.6 Calibration Curve of Standard
Description: NONE
Experiment Description: Cells from line 8226 were lysed in 8M urea/100mM ammonium bicarbonate and then were subjected to in-solution digestion with trypsin, following reduction and alkylation. The digest was then diluted down to the equivalent of 500 cells and injected concurrently with internal standards into a Thermo Scientific TSQ mass spectrometer.
2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times
2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides
