Information for Peptide: FMQVVVSR from MET_HUMAN

1. Biological Peptide

1.1 Native Sequence

    R.FMQVVVSR.S

1.2 Peptide Properties

Start Stop MW m/z for 2+ m/z for 3+ pI
462 469 964.52 483.27 322.51 9.75

1.3 Fragment Ion Table

AA b Ion Series b Ion Mass y Ion Series y Ion Mass
F b y
M b y
Q b y
V b y
V b y
V b y
S b y
R b y

2. Synthetic Peptide

2.1 Synthetic (Internal Standard) Peptide Sequence

(NONE)

2.2 Peptide Properties

(NONE)

2.3 Fragment Ion Table

(NONE)

2.4 Peptide Synthesis Report

(NONE)

2.5 QqQ MS/MS

2.6 Calibration Curve of Standard

Description: NONE

 

Experiment Description: Cells from colon cancer line HCT116 AND HT29 and Breast cancer cell line SKBR3 were lysed in 8M urea/100mM Ammonium bicarbonate buffer. The equivalent of 200,000 cells were loaded onto an SDS gel.

2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times

2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides