Information for Peptide: EPQVYTLPPSR from IGHG3_HUMAN
1. Biological Peptide
1.1 Native Sequence
R.EPQVYTLPPSR.E
1.2 Peptide Properties
| Start | Stop | MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|---|---|
| 275 | 285 | 1285.67 | 643.84 | 429.56 | 6.1 |
1.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| E | b | y | ||
| P | b | y | ||
| Q | b | y | ||
| V | b | y | ||
| Y | b | y | ||
| T | b | y | ||
| L | b | y | ||
| P | b | y | ||
| P | b | y | ||
| S | b | y | ||
| R | b | y |
2. Synthetic Peptide
2.1 Synthetic (Internal Standard) Peptide Sequence
DPQVYTLPPSR (Modification: E1 to D )
2.2 Peptide Properties
| MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|
| 1271.65 | 636.83 | 424.89 | 5.84 |
2.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| D | b | y | ||
| P | b | y | ||
| Q | b | y | ||
| V | b | y | ||
| Y | b | y | ||
| T | b | y | ||
| L | b | y | ||
| P | b | y | ||
| P | b | y | ||
| S | b | y | ||
| R | b | y |
2.4 Peptide Synthesis Report
Click to view/download synthesis report for EPQVYTLPPSR
2.5 QqQ MS/MS
Transition Table:
| Transition | m/z of Transition | Collision Energy |
|---|---|---|
| y4 | 456.257 | 25.0 |
| y6 | 670.389 | 25.0 |
| y7 | 833.452 | 25.0 |
| y9 | 932.52 | 25.0 |
2.6 Calibration Curve of Standard
Description: Internal standard DPQVYTLPPSR was diluted from 50 fmol to 500 amol injection concentrations and was found to be sensitive down to 5 fmol
Experiment Description: Serum sample digest from healthy adult venous blood draw. Sample denatured in Urea, reduced and alkylated (20mM TCEP and 200mM IAA) and digested overnight with trypsin. Sample was diluted in 2% ACN with 0.1% formic acid and a total of 0.1 nanoliters of serum was injected onto column.
2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times
2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides
