Information for Peptide: EPAAQAPVK from IGHD_HUMAN
1. Biological Peptide
1.1 Native Sequence
R.EPAAQAPVK.L
1.2 Peptide Properties
| Start | Stop | MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|---|---|
| 266 | 274 | 909.49 | 455.75 | 304.17 | 6.1 |
1.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| E | b | y | ||
| P | b | y | ||
| A | b | y | ||
| A | b | y | ||
| Q | b | y | ||
| A | b | y | ||
| P | b | y | ||
| V | b | y | ||
| K | b | y |
2. Synthetic Peptide
2.1 Synthetic (Internal Standard) Peptide Sequence
EPAGQAPVK (Modification: A4 to G )
2.2 Peptide Properties
| MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|
| 895.48 | 448.75 | 299.5 | 6.1 |
2.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| E | b | y | ||
| P | b | y | ||
| A | b | y | ||
| G | b | y | ||
| Q | b | y | ||
| A | b | y | ||
| P | b | y | ||
| V | b | y | ||
| K | b | y |
2.4 Peptide Synthesis Report
Click to view/download synthesis report for EPAAQAPVK
2.5 QqQ MS/MS
Transition Table:
| Transition | m/z of Transition | Collision Energy |
|---|---|---|
| y5 | 542.33 | 19.0 |
| y6 | 599.351 | 19.0 |
| y7 | 670.388 | 19.0 |
2.6 Calibration Curve of Standard
Description: NONE
Experiment Description: Serum sample digest from IgD myeloma patients venous blood draw. Sample denatured in Urea, reduced and alkylated (20mM TCEP and 200mM IAA) and digested overnight with trypsin. Sample was diluted in 2% ACN with 0.1% formic acid and a total of 0.1 nanoliters of serum was injected onto column.
2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times
2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides
