Information for Peptide: EIAEAYLGGK from HSP72_HUMAN
1. Biological Peptide
1.1 Native Sequence
K.EIAEAYLGGK.V
1.2 Peptide Properties
| Start | Stop | MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|---|---|
| 130 | 139 | 1049.54 | 525.78 | 350.85 | 4.53 |
1.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| E | b | y | ||
| I | b | y | ||
| A | b | y | ||
| E | b | y | ||
| A | b | y | ||
| Y | b | y | ||
| L | b | y | ||
| G | b | y | ||
| G | b | y | ||
| K | b | y |
2. Synthetic Peptide
2.1 Synthetic (Internal Standard) Peptide Sequence
EIAEAYL(C6N1)GGK (Modification: Stable isotope on L7 (U-13C, 15N) )
2.2 Peptide Properties
| MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|
| 1056.56 | 529.29 | 353.19 | 4.53 |
2.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| E | b | y | ||
| I | b | y | ||
| A | b | y | ||
| E | b | y | ||
| A | b | y | ||
| Y | b | y | ||
| L | b | y | ||
| G | b | y | ||
| G | b | y | ||
| K | b | y |
2.4 Peptide Synthesis Report
Click to view/download synthesis report for EIAEAYLGGK
2.5 QqQ MS/MS
Transition Table:
| Transition | m/z of Transition | Collision Energy |
|---|---|---|
| y4 | 381.24039 | 23.0 |
| y5 | 544.30372 | 23.0 |
| y6 | 615.34083 | 23.0 |
| y7 | 744.38342 | 23.0 |
| y8 | 815.42054 | 23.0 |
2.6 Calibration Curve of Standard
Description: NONE
Experiment Description: Cells from AML line U937 were lysed in 8M urea/ 100mM ammonium bicarbonate buffer on ice. The equivalent of 100,000 cells were loaded onto a SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/4 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer.
2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times
2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides
