Information for Peptide: EGYVPSNFVAR from BLK_HUMAN
1. Biological Peptide
1.1 Native Sequence
R.EGYVPSNFVAR.V
1.2 Peptide Properties
| Start | Stop | MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|---|---|
| 105 | 115 | 1237.61 | 619.81 | 413.54 | 6.1 |
1.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| E | b | y | ||
| G | b | y | ||
| Y | b | y | ||
| V | b | y | ||
| P | b | y | ||
| S | b | y | ||
| N | b | y | ||
| F | b | y | ||
| V | b | y | ||
| A | b | y | ||
| R | b | y |
2. Synthetic Peptide
2.1 Synthetic (Internal Standard) Peptide Sequence
EGYVPSNFV(c5n1)AR (Modification: Stable Isotope on Y9 (U-13C5, 15N1) )
2.2 Peptide Properties
| MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|
| 1243.62 | 622.82 | 415.55 | 6.1 |
2.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| E | b | y | ||
| G | b | y | ||
| Y | b | y | ||
| V | b | y | ||
| P | b | y | ||
| S | b | y | ||
| N | b | y | ||
| F | b | y | ||
| V | b | y | ||
| A | b | y | ||
| R | b | y |
2.4 Peptide Synthesis Report
Click to view/download synthesis report for EGYVPSNFVAR
2.5 QqQ MS/MS
Transition Table:
| Transition | m/z of Transition | Collision Energy |
|---|---|---|
| y9 | 1058.57 | 26.0 |
| y8 | 895.5 | 20.0 |
| y7 | 796.43 | 21.0 |
| y6 | 699.38 | 31.0 |
2.6 Calibration Curve of Standard
Description: Internal standard was diluted from 50 fmol to 500 amol injection concentrations and was found to be sensitive down to 0.1 fmol
Experiment Description: Cells from line 8226 were lysed in RIPA buffer. The equivalent of 50,000 cells were loaded onto an SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/4 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer.
2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times
2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides
