Information for Peptide: EGLLAIFK from CTNB1_HUMAN
1. Biological Peptide
1.1 Native Sequence
R.EGLLAIFK.S
1.2 Peptide Properties
| Start | Stop | MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|---|---|
| 226 | 233 | 889.53 | 445.77 | 297.52 | 6.1 |
1.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| E | b | y | ||
| G | b | y | ||
| L | b | y | ||
| L | b | y | ||
| A | b | y | ||
| I | b | y | ||
| F | b | y | ||
| K | b | y |
2. Synthetic Peptide
2.1 Synthetic (Internal Standard) Peptide Sequence
DGLLAIFK (Modification: E1 to D )
2.2 Peptide Properties
| MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|
| 875.51 | 438.76 | 292.85 | 5.84 |
2.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| D | b | y | ||
| G | b | y | ||
| L | b | y | ||
| L | b | y | ||
| A | b | y | ||
| I | b | y | ||
| F | b | y | ||
| K | b | y |
2.4 Peptide Synthesis Report
Click to view/download synthesis report for EGLLAIFK
2.5 QqQ MS/MS
Transition Table:
| Transition | m/z of Transition | Collision Energy |
|---|---|---|
| y7 | 761.49 | 19.0 |
| y6 | 704.47 | 16.0 |
| y5 | 591.39 | 15.0 |
| y4 | 478.3 | 14.0 |
| y3 | 407.27 | 18.0 |
| y2 | 294.18 | 23.0 |
2.6 Calibration Curve of Standard
Description: Dilution curve was performed from 50 fmol to 0.1 fmol. Peptide was detedtable to 10 fmol.
Experiment Description: Cells from colon caner line HCT 116 were lysed in 8M urea/ 100mM ammonium bicarbonate buffer on ice. The equivalent of 100,000 cells were loaded onto a SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/4 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer.
2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times
2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides
