Information for Peptide: EGEEAGPGDPLLEAVPK from CDC37_HUMAN
1. Biological Peptide
1.1 Native Sequence
K.EGEEAGPGDPLLEAVPK.T
1.2 Peptide Properties
| Start | Stop | MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|---|---|
| 353 | 369 | 1706.84 | 854.43 | 569.95 | 3.91 |
1.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| E | b | y | ||
| G | b | y | ||
| E | b | y | ||
| E | b | y | ||
| A | b | y | ||
| G | b | y | ||
| P | b | y | ||
| G | b | y | ||
| D | b | y | ||
| P | b | y | ||
| L | b | y | ||
| L | b | y | ||
| E | b | y | ||
| A | b | y | ||
| V | b | y | ||
| P | b | y | ||
| K | b | y |
2. Synthetic Peptide
2.1 Synthetic (Internal Standard) Peptide Sequence
EGEEAGPGDPLLEAVP(C5N1)K (Modification: Stable Isotope on P16 (U-13C5, 15N) )
2.2 Peptide Properties
| MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|
| 1712.85 | 857.43 | 571.96 | 3.91 |
2.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| E | b | y | ||
| G | b | y | ||
| E | b | y | ||
| E | b | y | ||
| A | b | y | ||
| G | b | y | ||
| P | b | y | ||
| G | b | y | ||
| D | b | y | ||
| P | b | y | ||
| L | b | y | ||
| L | b | y | ||
| E | b | y | ||
| A | b | y | ||
| V | b | y | ||
| P | b | y | ||
| K | b | y |
2.4 Peptide Synthesis Report
Click to view/download synthesis report for EGEEAGPGDPLLEAVPK
2.5 QqQ MS/MS
Transition Table:
| Transition | m/z of Transition | Collision Energy |
|---|---|---|
| y4 | 420.27 | 32.0 |
| y5 | 549.31 | 32.0 |
| y6 | 662.4 | 32.0 |
| y7 | 775.48 | 32.0 |
| y8 | 872.54 | 32.0 |
| y9 | 987.56 | 32.0 |
2.6 Calibration Curve of Standard
Description: NONE
Experiment Description: Cells from AML line U937 were lysed in 8M urea/ 100mM ammonium bicarbonate buffer on ice. The lysate was digested insolution with trypsin after reduction and alkylation with TCEP and IAA.
2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times
2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides
