Information for Peptide: DWVSVVTPAR from UROM_HUMAN
1. Biological Peptide
1.1 Native Sequence
R.DWVSVVTPAR.D
1.2 Peptide Properties
| Start | Stop | MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|---|---|
| 373 | 382 | 1128.59 | 565.3 | 377.21 | 5.84 |
1.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| D | b | y | ||
| W | b | y | ||
| V | b | y | ||
| S | b | y | ||
| V | b | y | ||
| V | b | y | ||
| T | b | y | ||
| P | b | y | ||
| A | b | y | ||
| R | b | y |
2. Synthetic Peptide
2.1 Synthetic (Internal Standard) Peptide Sequence
DWVSVVTP(C5N1)AR (Modification: Stable Isotope label on P8 (U-13C, 15N) )
2.2 Peptide Properties
| MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|
| 1134.61 | 568.31 | 379.21 | 5.84 |
2.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| D | b | y | ||
| W | b | y | ||
| V | b | y | ||
| S | b | y | ||
| V | b | y | ||
| V | b | y | ||
| T | b | y | ||
| P | b | y | ||
| A | b | y | ||
| R | b | y |
2.4 Peptide Synthesis Report
Click to view/download synthesis report for DWVSVVTPAR
2.5 QqQ MS/MS
Transition Table:
| Transition | m/z of Transition | Collision Energy |
|---|---|---|
| y8 | 834.49437 | 25.0 |
| y7 | 735.42596 | 25.0 |
| y5 | 549.32551 | 25.0 |
| y4 | 450.2571 | 25.0 |
2.6 Calibration Curve of Standard
Description: NONE
Experiment Description: Control urine was processed via ultrafiltration according to Moffitt Proteomics protocol and subjected to in-solution digestion with trypsin, following thermal denaturation, reduction, and alkylation.
2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times
2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides
