Information for Peptide: DVNAAIATIK from TBA1C_HUMAN
1. Biological Peptide
1.1 Native Sequence
K.DVNAAIATIK.T
1.2 Peptide Properties
| Start | Stop | MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|---|---|
| 327 | 336 | 1014.57 | 508.29 | 339.2 | 5.84 |
1.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| D | b | y | ||
| V | b | y | ||
| N | b | y | ||
| A | b | y | ||
| A | b | y | ||
| I | b | y | ||
| A | b | y | ||
| T | b | y | ||
| I | b | y | ||
| K | b | y |
2. Synthetic Peptide
2.1 Synthetic (Internal Standard) Peptide Sequence
DVNAAIGTIK (Modification: A7 to G )
2.2 Peptide Properties
| MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|
| 1000.56 | 501.29 | 334.53 | 5.84 |
2.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| D | b | y | ||
| V | b | y | ||
| N | b | y | ||
| A | b | y | ||
| A | b | y | ||
| I | b | y | ||
| G | b | y | ||
| T | b | y | ||
| I | b | y | ||
| K | b | y |
2.4 Peptide Synthesis Report
Click to view/download synthesis report for DVNAAIATIK
2.5 QqQ MS/MS
Transition Table:
| Transition | m/z of Transition | Collision Energy |
|---|---|---|
| b3 | 329.14615 | 15.0 |
| b4 | 400.18327 | 15.0 |
| b6 | 584.30444 | 13.0 |
| y3 | 361.24514 | 27.0 |
| y4 | 418.2666 | 18.0 |
| y5 | 531.35067 | 21.0 |
| y6 | 602.38778 | 18.0 |
| y7 | 673.42489 | 18.0 |
| y8 | 787.46782 | 17.0 |
2.6 Calibration Curve of Standard
Description: NONE
Experiment Description: Cells from line 8226 were lysed in 8M urea/100mM ammonium bicarbonate and then were subjected to in-solution digestion with trypsin, following reduction and alkylation. The digest was then diluted down to the equivalent of 500 cells and injected concurrently with internal standards into a Thermo Scientific TSQ mass spectrometer.
2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times
2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides
