Information for Peptide: DLPHITVDR from MMP7_HUMAN

1. Biological Peptide

1.1 Native Sequence

    R.DLPHITVDR.L

1.2 Peptide Properties

Start	Stop	MW	m/z for 2+	m/z for 3+	pI
119	127	1064.56	533.29	355.86	5.21

1.3 Fragment Ion Table

AA	b Ion Series	b Ion Mass	y Ion Series	y Ion Mass
D	b		y
L	b		y
P	b		y
H	b		y
I	b		y
T	b		y
V	b		y
D	b		y
R	b		y

2. Synthetic Peptide

2.1 Synthetic (Internal Standard) Peptide Sequence

DLPHITV(c5N1)DR (Modification: Stable Isotope on V7 (U-13C5, 15N) )

2.2 Peptide Properties

MW	m/z for 2+	m/z for 3+	pI
1070.58	536.3	357.87	5.21

2.3 Fragment Ion Table

AA	b Ion Series	b Ion Mass	y Ion Series	y Ion Mass
D	b		y
L	b		y
P	b		y
H	b		y
I	b		y
T	b		y
V	b		y
D	b		y
R	b		y

2.4 Peptide Synthesis Report

Click to view/download synthesis report for DLPHITVDR

2.5 QqQ MS/MS

Transition Table:

Transition	m/z of Transition	Collision Energy
y3	395.21	22.0
y4	496.26	22.0
y5	609.35	22.0

2.6 Calibration Curve of Standard

Description: A Dilution series was done on the internal standard from 50 fmol to 0.1 fmol. Sensitivity was determined down to 1 fmol.

 

Experiment Description: Cells from colon caner line HCT 116 were lysed in 8M urea/ 100mM ammonium bicarbonate buffer on ice. The equivalent of 100,000 cells were loaded onto a SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/4 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer. Internal standard has been synthesized and is awaiting analysis with sample.

2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times

2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides