Information for Peptide: DDSPDLPK from ALBU_BOVIN
1. Biological Peptide
1.1 Native Sequence
K.DDSPDLPK.L
1.2 Peptide Properties
| Start | Stop | MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|---|---|
| 131 | 138 | 885.41 | 443.71 | 296.14 | 3.93 |
1.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| D | b | y | ||
| D | b | y | ||
| S | b | y | ||
| P | b | y | ||
| D | b | y | ||
| L | b | y | ||
| P | b | y | ||
| K | b | y |
2. Synthetic Peptide
2.1 Synthetic (Internal Standard) Peptide Sequence
DESPDLPK (Modification: D2 to E )
2.2 Peptide Properties
| MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|
| 899.42 | 450.72 | 300.82 | 4.03 |
2.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| D | b | y | ||
| E | b | y | ||
| S | b | y | ||
| P | b | y | ||
| D | b | y | ||
| L | b | y | ||
| P | b | y | ||
| K | b | y |
2.4 Peptide Synthesis Report
Click to view/download synthesis report for DDSPDLPK
2.5 QqQ MS/MS
Transition Table:
| Transition | m/z of Transition | Collision Energy |
|---|---|---|
| y4 | 472.276575 | 19.0 |
| y5 | 569.329339 | 19.0 |
| y6 | 656.361367 | 19.0 |
2.6 Calibration Curve of Standard
Description: A dilution series was done from 10 fmoles per injection to 0.2 fmoles. Detection was possible throughout the series.
Experiment Description: 10fmol of BSA standard was analysed on the TSQ. Sample was analyzed concurrently with synthetic internal standards
2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times
2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides
