Information for Peptide: AVGPEITK from 2AAA_HUMAN
1. Biological Peptide
1.1 Native Sequence
K.AVGPEITK.T
1.2 Peptide Properties
| Start | Stop | MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|---|---|
| 273 | 280 | 813.46 | 407.74 | 272.16 | 6.05 |
1.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| A | b | y | ||
| V | b | y | ||
| G | b | y | ||
| P | b | y | ||
| E | b | y | ||
| I | b | y | ||
| T | b | y | ||
| K | b | y |
2. Synthetic Peptide
2.1 Synthetic (Internal Standard) Peptide Sequence
GVGPEITK (Modification: A1 to G )
2.2 Peptide Properties
| MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|
| 799.44 | 400.73 | 267.49 | 6.0 |
2.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| G | b | y | ||
| V | b | y | ||
| G | b | y | ||
| P | b | y | ||
| E | b | y | ||
| I | b | y | ||
| T | b | y | ||
| K | b | y |
2.4 Peptide Synthesis Report
Click to view/download synthesis report for AVGPEITK
2.5 QqQ MS/MS
Transition Table:
| Transition | m/z of Transition | Collision Energy |
|---|---|---|
| y3 | 361.25 | 19.0 |
| y4 | 490.29 | 17.0 |
| y5 | 587.34 | 13.0 |
| y6 | 644.36 | 12.0 |
2.6 Calibration Curve of Standard
Description: Internal standard was diluted from 50 fmol to 500 amol injection concentrations and was found to be sensitive down to 0.1 fmol
Experiment Description: Cells from colon caner line HCT 116 were lysed in 8M urea/ 100mM ammonium bicarbonate buffer on ice. The equivalent of 100,000 cells were loaded onto a SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/4 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer. Sample was analyzed concurrently with internal standard
2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times
2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides
