Information for Peptide: ANFENLAK from SRC8_HUMAN

1. Biological Peptide

1.1 Native Sequence

    R.ANFENLAK.E

1.2 Peptide Properties

Start	Stop	MW	m/z for 2+	m/z for 3+	pI
352	359	905.46	453.74	302.83	6.05

1.3 Fragment Ion Table

AA	b Ion Series	b Ion Mass	y Ion Series	y Ion Mass
A	b		y
N	b		y
F	b		y
E	b		y
N	b		y
L	b		y
A	b		y
K	b		y

2. Synthetic Peptide

2.1 Synthetic (Internal Standard) Peptide Sequence

ANFENL(C6N1)AK (Modification: Stable Isotope on L6 (U-13C6, 15N) )

2.2 Peptide Properties

MW	m/z for 2+	m/z for 3+	pI
912.48	457.25	305.17	6.05

2.3 Fragment Ion Table

AA	b Ion Series	b Ion Mass	y Ion Series	y Ion Mass
A	b		y
N	b		y
F	b		y
E	b		y
N	b		y
L	b		y
A	b		y
K	b		y

2.4 Peptide Synthesis Report

Click to view/download synthesis report for ANFENLAK

2.5 QqQ MS/MS

Transition Table:

Transition	m/z of Transition	Collision Energy
y4	452.2775	19.0
y5	581.32009	19.0
y6	728.38851	19.0
y7	842.43144	19.0

2.6 Calibration Curve of Standard

Description: A dilution series was done on the internal standard peptide from 50 Fmol to 0.1 fmol. Peptide detection was possible to 0.1 fmol.

 

Experiment Description: Cells from colon caner line HCT 116 were lysed in 8M urea/ 100mM ammonium bicarbonate buffer on ice. The equivalent of 100,000 cells were loaded onto a SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/4 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer. Sample was analyzed concurrently with internal standard

2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times

2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides