Information for Peptide: ALGDELHQR from HRK_HUMAN

1. Biological Peptide

1.1 Native Sequence

    K.ALGDELHQR.T

1.2 Peptide Properties

Start	Stop	MW	m/z for 2+	m/z for 3+	pI
39	47	1037.53	519.77	346.85	5.32

1.3 Fragment Ion Table

AA	b Ion Series	b Ion Mass	y Ion Series	y Ion Mass
A	b		y
L	b		y
G	b		y
D	b		y
E	b		y
L	b		y
H	b		y
Q	b		y
R	b		y

2. Synthetic Peptide

2.1 Synthetic (Internal Standard) Peptide Sequence

ALGDEL(c6N1)HQR (Modification: Stable Isotope on L6 (U-13C6, 15N) )

2.2 Peptide Properties

MW	m/z for 2+	m/z for 3+	pI
1044.54	523.28	349.19	5.32

2.3 Fragment Ion Table

AA	b Ion Series	b Ion Mass	y Ion Series	y Ion Mass
A	b		y
L	b		y
G	b		y
D	b		y
E	b		y
L	b		y
H	b		y
Q	b		y
R	b		y

2.4 Peptide Synthesis Report

Click to view/download synthesis report for ALGDELHQR

2.5 QqQ MS/MS

Transition Table:

Transition	m/z of Transition	Collision Energy
y8	974.51	24.0
y7	861.43	22.0
y6	804.41	24.0
y5	689.38	24.0
y4	560.34	26.0

2.6 Calibration Curve of Standard

Description: A dilution series was done in the internal standard with injection concentrations from 0.1 fmole to 50 fmole. Sensitivity was determined to 0.1 fmole.

 

Experiment Description: Cells from 8226 were lysed in RIPA buffer on ice. The equivalent of 100,000 cells were loaded onto a SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/4 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer. Sample is awaiting analysis concurrently with internal standard

2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times

2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides