Information for Peptide: ALDFAVGEYNK from CYTC_HUMAN
1. Biological Peptide
1.1 Native Sequence
R.ALDFAVGEYNK.A
1.2 Peptide Properties
| Start | Stop | MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|---|---|
| 52 | 62 | 1225.6 | 613.81 | 409.54 | 4.37 |
1.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| A | b | y | ||
| L | b | y | ||
| D | b | y | ||
| F | b | y | ||
| A | b | y | ||
| V | b | y | ||
| G | b | y | ||
| E | b | y | ||
| Y | b | y | ||
| N | b | y | ||
| K | b | y |
2. Synthetic Peptide
2.1 Synthetic (Internal Standard) Peptide Sequence
ALDFAVGDYNK (Modification: Amino acid switch of E8 to D )
2.2 Peptide Properties
| MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|
| 1211.58 | 606.8 | 404.87 | 4.21 |
2.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| A | b | y | ||
| L | b | y | ||
| D | b | y | ||
| F | b | y | ||
| A | b | y | ||
| V | b | y | ||
| G | b | y | ||
| D | b | y | ||
| Y | b | y | ||
| N | b | y | ||
| K | b | y |
2.4 Peptide Synthesis Report
Click to view/download synthesis report for ALDFAVGEYNK
2.5 QqQ MS/MS
Transition Table:
| Transition | m/z of Transition | Collision Energy |
|---|---|---|
| y9 | 1028.46894 | 24.0 |
| y8 | 913.442 | 24.0 |
| y7 | 766.37359 | 24.0 |
| y6 | 695.33647 | 24.0 |
2.6 Calibration Curve of Standard
Description: A dilution series was done on the internal standard peptide from 50 Fmol to 0.1 fmol. Peptide detection was possible to 0.1 fmol.
Experiment Description: Control urine was processed via ultrafiltration according to Moffitt Proteomics protocol and subjected to in-solution digestion with trypsin, following thermal denaturation, reduction, and alkylation.
2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times
2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides
