Information for Peptide: AIPELTK from CTNB1_HUMAN
1. Biological Peptide
1.1 Native Sequence
R.AIPELTK.L
1.2 Peptide Properties
| Start | Stop | MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|---|---|
| 152 | 158 | 770.45 | 386.23 | 257.83 | 6.05 |
1.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| A | b | y | ||
| I | b | y | ||
| P | b | y | ||
| E | b | y | ||
| L | b | y | ||
| T | b | y | ||
| K | b | y |
2. Synthetic Peptide
2.1 Synthetic (Internal Standard) Peptide Sequence
AIPEL(C6N1)TK (Modification: Stable Isotope on L5 (U-13C6, 15N) )
2.2 Peptide Properties
| MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|
| 777.47 | 389.74 | 260.16 | 6.05 |
2.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| A | b | y | ||
| I | b | y | ||
| P | b | y | ||
| E | b | y | ||
| L | b | y | ||
| T | b | y | ||
| K | b | y |
2.4 Peptide Synthesis Report
Click to view/download synthesis report for AIPELTK
2.5 QqQ MS/MS
Transition Table:
| Transition | m/z of Transition | Collision Energy |
|---|---|---|
| y3 | 368.24514 | 17.0 |
| y4 | 497.28773 | 17.0 |
| y5 | 594.3405 | 17.0 |
| y6 | 707.42456 | 17.0 |
2.6 Calibration Curve of Standard
Description: Dilution curve was performed from 50 fmol to 0.1 fmol. Peptide was detectable to 0.10 fmol.
Experiment Description: Cells from colon caner line HCT 116 were lysed in 8M urea/ 100mM ammonium bicarbonate buffer on ice. The equivalent of 100,000 cells were loaded onto a SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/4 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer. Sample was analyzed concurrently with internal standard
2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times
2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides
