Information for Peptide: AGVETTTPSK from LAC_HUMAN
1. Biological Peptide
1.1 Native Sequence
K.AGVETTTPSK.Q
1.2 Peptide Properties
| Start | Stop | MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|---|---|
| 50 | 59 | 989.5 | 495.76 | 330.84 | 6.05 |
1.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| A | b | y | ||
| G | b | y | ||
| V | b | y | ||
| E | b | y | ||
| T | b | y | ||
| T | b | y | ||
| T | b | y | ||
| P | b | y | ||
| S | b | y | ||
| K | b | y |
2. Synthetic Peptide
2.1 Synthetic (Internal Standard) Peptide Sequence
AGVETTTP(C5N1)SK (Modification: Stable Isotope P(U-13C5, 15N) )
2.2 Peptide Properties
| MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|
| 995.52 | 498.77 | 332.85 | 6.05 |
2.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| A | b | y | ||
| G | b | y | ||
| V | b | y | ||
| E | b | y | ||
| T | b | y | ||
| T | b | y | ||
| T | b | y | ||
| P | b | y | ||
| S | b | y | ||
| K | b | y |
2.4 Peptide Synthesis Report
Click to view/download synthesis report for AGVETTTPSK
2.5 QqQ MS/MS
Transition Table:
| Transition | m/z of Transition | Collision Energy |
|---|---|---|
| y5 | 539.29 | 20.0 |
| y6 | 640.34 | 20.0 |
| y7 | 769.38 | 20.0 |
2.6 Calibration Curve of Standard
Description: Peptide was diluted from 50 fmol down to 0.1 fmol and was detectale down to the lowest value
Experiment Description: Serum sample digest from healthy adult venous blood draw. Sample denatured in Urea, reduced and alkylated (20mM TCEP and 200mM IAA) and digested overnight with trypsin. Sample was diluted in 2% ACN with 0.1% formic acid and a total of 0.1 nanoliters of serum was injected onto column.
2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times
2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides
