Information for Peptide: AGFAGDDAPR from ACTB_HUMAN
1. Biological Peptide
1.1 Native Sequence
K.AGFAGDDAPR.A
1.2 Peptide Properties
| Start | Stop | MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|---|---|
| 19 | 28 | 975.44 | 488.73 | 326.15 | 4.21 |
1.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| A | b | y | ||
| G | b | y | ||
| F | b | y | ||
| A | b | y | ||
| G | b | y | ||
| D | b | y | ||
| D | b | y | ||
| A | b | y | ||
| P | b | y | ||
| R | b | y |
2. Synthetic Peptide
2.1 Synthetic (Internal Standard) Peptide Sequence
AGFAGDDAP(c6n1)R (Modification: Stable Isotope on P9 (U-13C6, 15N) )
2.2 Peptide Properties
| MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|
| 982.46 | 492.24 | 328.49 | 4.21 |
2.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| A | b | y | ||
| G | b | y | ||
| F | b | y | ||
| A | b | y | ||
| G | b | y | ||
| D | b | y | ||
| D | b | y | ||
| A | b | y | ||
| P | b | y | ||
| R | b | y |
2.4 Peptide Synthesis Report
Click to view/download synthesis report for AGFAGDDAPR
2.5 QqQ MS/MS
Transition Table:
| Transition | m/z of Transition | Collision Energy |
|---|---|---|
| y7 | 707.32189 | 18.0 |
| y8 | 854.3903 | 24.0 |
| y9 | 911.41176 | 24.0 |
| y10 | 982.44888 | 24.0 |
2.6 Calibration Curve of Standard
Description: NONE
Experiment Description: Cells from HCT116 were lysed in 8M urea/ 100mM ammonium bicarbonate buffer on ice. The equivalent of 100,000 cells were loaded onto a SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/4 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer.
2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times
2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides
