Information for Peptide: AFSDPFVEAEK from HSP74_HUMAN
1. Biological Peptide
1.1 Native Sequence
R.AFSDPFVEAEK.S
1.2 Peptide Properties
| Start | Stop | MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|---|---|
| 74 | 84 | 1238.58 | 620.3 | 413.87 | 4.14 |
1.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| A | b | y | ||
| F | b | y | ||
| S | b | y | ||
| D | b | y | ||
| P | b | y | ||
| F | b | y | ||
| V | b | y | ||
| E | b | y | ||
| A | b | y | ||
| E | b | y | ||
| K | b | y |
2. Synthetic Peptide
2.1 Synthetic (Internal Standard) Peptide Sequence
AFSDPFV(C5N1)EAEK (Modification: Stable isotope on V7 (U-13C, 15N) )
2.2 Peptide Properties
| MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|
| 1244.6 | 623.31 | 415.87 | 4.14 |
2.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| A | b | y | ||
| F | b | y | ||
| S | b | y | ||
| D | b | y | ||
| P | b | y | ||
| F | b | y | ||
| V | b | y | ||
| E | b | y | ||
| A | b | y | ||
| E | b | y | ||
| K | b | y |
2.4 Peptide Synthesis Report
Click to view/download synthesis report for AFSDPFVEAEK
2.5 QqQ MS/MS
Transition Table:
| Transition | m/z of Transition | Collision Energy |
|---|---|---|
| y7 | 1174.55267 | 22.0 |
| y8 | 1027.48426 | 22.0 |
| y9 | 940.45223 | 22.0 |
| y10 | 825.42529 | 22.0 |
2.6 Calibration Curve of Standard
Description: NONE
Experiment Description: Cells from AML line U937 were lysed in 8M urea/ 100mM ammonium bicarbonate buffer on ice. The equivalent of 100,000 cells were loaded onto a SDS gel. The protein of interest was excised and ingel digestion with trypsin was performed after reduction and alkylation with TCEP and IAA. 1/4 of the resulting digest was then analyzed on a Thermo Scientific TSQ mass spectrometer.
2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times
2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides
