Information for Peptide: AEFVEVTK from ALBU_BOVIN
1. Biological Peptide
1.1 Native Sequence
K.AEFVEVTK.L
1.2 Peptide Properties
| Start | Stop | MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|---|---|
| 249 | 256 | 921.48 | 461.75 | 308.17 | 4.53 |
1.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| A | b | y | ||
| E | b | y | ||
| F | b | y | ||
| V | b | y | ||
| E | b | y | ||
| V | b | y | ||
| T | b | y | ||
| K | b | y |
2. Synthetic Peptide
2.1 Synthetic (Internal Standard) Peptide Sequence
AEFVEV(C5N1)TK (Modification: Stable Isotope label on V6 (U-13C5, 15N1) )
2.2 Peptide Properties
| MW | m/z for 2+ | m/z for 3+ | pI |
|---|---|---|---|
| 927.49 | 464.76 | 310.17 | 4.53 |
2.3 Fragment Ion Table
| AA | b Ion Series | b Ion Mass | y Ion Series | y Ion Mass |
|---|---|---|---|---|
| A | b | y | ||
| E | b | y | ||
| F | b | y | ||
| V | b | y | ||
| E | b | y | ||
| V | b | y | ||
| T | b | y | ||
| K | b | y |
2.4 Peptide Synthesis Report
Click to view/download synthesis report for AEFVEVTK
2.5 QqQ MS/MS
Transition Table:
| Transition | m/z of Transition | Collision Energy |
|---|---|---|
| y4 | 482.285298 | 19.0 |
| y5 | 581.353712 | 19.0 |
| y6 | 728.422126 | 19.0 |
2.6 Calibration Curve of Standard
Description: A dilution series was done from 10 fmoles per injection to 0.2 fmoles. Detection was possible throughout the series.
Experiment Description: 10fmol of BSA standard was analysed on the TSQ. Sample was analyzed concurrently with synthetic internal standards
2.7 LC-MRM Analysis of Biological and Standard Peptides Illustrates Elution Times
2.8 Pseudo MS/MS Comparison of Transition Patterns for Biological/Standard Peptides
